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Thermo Fisher
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ABclonal Biotechnology
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GeneTex
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Journal: Discover Oncology
Article Title: Alterations of drug-resistant proteins in nasopharyngeal carcinoma after intensity-modulated radiation therapy with or without chemotherapy and correlation with clinicopathologic features and short-term prognosis
doi: 10.1007/s12672-025-02677-y
Figure Lengend Snippet: Short-term PFS of NPC patients based on post-treatment resistant protein expression in positive and negative subgroups. A P-gp Positive and P-gp Negative; B MRP Positive and MRP Negative; C LRP Positive and LRP Negative; D GST- π Positive and GST-π Negative. log-rank < 0.05 was statistically significant
Article Snippet: At 4 °C, rat anti-human primary antibodies P-gp, MRP, LRP, and
Techniques: Expressing
Journal: Oncology Letters
Article Title: Potential antitumor effect of polysaccharides extracted from Polygonatum sibiricum on human prostate cancer PC‑3 cells
doi: 10.3892/ol.2024.14774
Figure Lengend Snippet: PSP decreases the expression of the MDR-1 gene and P-gp protein in PC-3 cells. PC-3 cells were treated with different concentrations of PSP for 72 h. (A) Reverse transcription-quantitative PCR was performed to detect the expression of the MDR-1 gene. At a concentration of 4 mg/ml, MDR-1 gene expression was significantly lower compared with at other concentrations. (B) Treated PC-3 cell slides were prepared and immunocytochemistry was used to assess the expression of P-gp, which demonstrated that the expression decreased gradually with higher PSP concentrations (magnification, ×400 scale bar=50 µm). **P<0.01. PSP, Polygonatum sibiricum ; MDR-1, multidrug resistance-1; P-gp, P-glycoprotein.
Article Snippet: The slides were subsequently stained with
Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Concentration Assay, Gene Expression, Immunocytochemistry